Polyvinyl alcohol scaffolds and supplementation support 3D and sphere culturing of human cancer cell lines by reducing apoptosis and promoting cellular proliferation

Three-dimensional (3D) culturing mimics the heterogeneous cellular conditions of in vivo tumor microenvironment compared to 2D monolayer-cultured cells and 3D cultures established cancer cell lines (sphere culture) or patient-derived (organoid are frequently used for research drug screening evaluation. To establish more cost time-efficient culture methods lines, we supplemented sphere medium with polyvinyl alcohol (PVA) found that breast pancreatic were significantly increased. Mechanistically, PVA prevented death promoted proliferation while maintaining levels stemness-related gene expression. Furthermore, showed formal resin (PVF) scaffolds made by cross-linked can function serum-free, long-term support maintenance sphere- tumor-like masses diverse types. Taken together, demonstrate effectiveness PVF human line protocols.